MOLECULAR EPIDEMIOLOGY OF Vibrio spp ISOLATED FROM ABATTOIR EFFLUENTS DISCHARGE INTO THE IKPOBA RIVER, BENIN CITY, NIGERIA

Abstract

Disposal of untreated abattoir effluent into natural water bodies pose serious public health concern. Therefore, monitoring of the bacteriological quality of polluted water bodies is of utmost importance. This study investigated occurrence and antibiogram of Vibrio strains of public health significance associated with abattoir effluents discharged into the Ikpoba river, Benin City, Nigeria. Test samples were collected weekly for 6 weeks from four (4) sites: point source (PS), confluent point (CP), 500 m upstream (US) and 500 m downstream (DS) from point of CP contact with the Ikpoba river respectively. The physico-chemical (pH, temperature, turbidity, sulphate, nitrate, phosphate, BOD, TSS, and TDS) and microbiological qualities of the test samples were determined using standard methods. Isolates were identified using standard microbiological and molecular techniques. The antibiotic susceptibility profile of selected Vibrio isolates were also determined and interpreted according to the description of Clinical Laboratory Standard Institute (CLSI). pH ranged between 5.61 and 7.03, while temperature varied from 22�C to 30�C. The highest values for turbidity was >4000 NTU, while the lowest was

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urn:uuid:6eaea6b5-864a-430c-b117-c044d32d9a2b